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Literature summary extracted from

  • de Souza, F.; Sales, A.; Costa e Silva, P.; Bezerra, R.; de Medeiros e Silva, G.; de Araújo, J.; de Campos Takaki, G.; Porto, T.; Teixeira, J.; Porto, A.
    Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens (2016), Macromol. Res., 24, 587-595 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 2-mercaptoethanol 36.2% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 Co2+ 48.3% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 Cu2+ 50% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 EDTA 10.4% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 Fe2+ 6.82% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 K+ 98.6% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 Mg2+ 96.8% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 pepstatin A 48.2% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 phenylmethylsulfonyl fluoride 8.48% residual activity at 5 mM Bacillus amyloliquefaciens
3.4.24.72 Zn2+ 52.6% residual activity at 5 mM Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Ca2+ 110% activity at 5 mM Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Bacillus amyloliquefaciens
-
-
-
3.4.24.72 Bacillus amyloliquefaciens UFPEDA 485
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 fibrinogen + H2O the highest fibrinolytic activity and a degradation of blood clot in vitro of 62% are obtained in a medium with 2% (w/v) soybean flour and 1% (w/v) D-glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37°C Bacillus amyloliquefaciens fibrin + ?
-
 ?
3.4.24.72 fibrinogen + H2O the highest fibrinolytic activity and a degradation of blood clot in vitro of 62% are obtained in a medium with 2% (w/v) soybean flour and 1% (w/v) D-glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37°C Bacillus amyloliquefaciens UFPEDA 485 fibrin + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 fibrinolytic protease
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 37
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.72 37
-
 after 12 h of incubation, the enzyme remains stable at 4, 25 and 37°C, maintaining activities of 96.04, 95.88, and 98.26%, respectively, and maintains 58.84% of its activity at 40°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 7
-
-
 Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.72 7
-
 after incubation for 12 h, the enzyme retains 82.89% of its activity at pH 7.0, 66.03% at pH 8.0, and 50% at pH 6.0 and 9.0 after 8 h of incubation, respectively Bacillus amyloliquefaciens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.24.72 5
-
 at pH 7.0 and 37°C Bacillus amyloliquefaciens Cu2+